阴道外组织是由正常的人类来源的阴道上皮细胞和树突状细胞培养而成. 其高度分化的结构与体内组织相似，是女性卫生毒性研究的理想选择, 阴道护理, 和杀微生物剂产品. Four types of Epi阴道 are offered:
1) VEC-100 (Figure 1): An epithelial tissue containing epithelial VEC cells,
2) VLC-100(图1):含有上皮VEC和免疫活性树突状细胞的上皮组织,
3) VEC-100- ft(图2):全层的VEC-100，包括VEC上皮细胞和含有成纤维细胞的固有层, 和
4) VLC-100-FT(图2):vecc -100- ft的免疫功能版本，包括树突状细胞.
图1:H&E染色的组织学(福尔马林固定)横截面A) VEC-100体外重建包含正常人VEC细胞的上皮组织模型, B)阴道外植体组织. 在体外和体内的组织都显示有核的基底和基底上细胞层，然后是细胞核缺失和细胞充满糖原的层.
图2:H&组织学(福尔马林固定)全层阴道外组织横断面E染色. VEC-100- ft组织由培养在含有成纤维细胞的固有层(LP)样胶原基质上的VEC上皮细胞组成. VLC-100-FT由上皮层的VEC上皮细胞和树突状细胞组成，并在LP中包含成纤维细胞和树突状细胞.
图3:细胞角蛋白免疫染色的VEC-100组织的福尔马林固定横断面, CK13, CK14, & CK18. Basal cells are stained by CK14 和 supra-basal cells by CK13; no cells are stained by CK18.
图4:与含有常见杀精剂的制剂接触18小时后，阴道外全层(VEC-100-FT)组织模型的活力(A)和细胞因子释放(B), nonoxynol-9 (N9): a) PBS control, b) KY果冻(KY), c) KY有2% N9号(2% N9号), 和d) 0.1%的N9号PBS (0.1% N9). Note: As the tissue viability decreases, the IL-1β release increases.
Figure 5: HIV-1 infection of VLC-100 tissue following topical exposure (24hr) to 60,000 CPM of HIV-1 ADA or NL4-3 virus. After exposure, tissues were washed 3X 和 then cultured for an additional 24 hr. 然后提取细胞DNA，使用HIV-1 gag特异性引物对检测HIV-1转录本.
图6:RT - PCR显示toll样受体(TLR)表达:A)器官型阴道-宫颈外位(VEC)组织模型和B)人宫颈组织外植体.
杀菌剂检测，性病感染研究，女性卫生，炎症，药物输送
过敏测试
使用阴道外组织测量接触女性卫生用品和其他产品后的组织活力和细胞因子释放.
Epi阴道为女性产品安全性测试以及HIV-1和其他性传播疾病的研究提供了一种体外研究工具. 其组织结构和细胞生理机能与体内阴道上皮组织紧密平行, making it ideal for toxicology 和 efficacy testing, as well as a tool for biological research. 浏览我们的 参考图书馆 to see how researchers have used our Epi阴道 tissue in these areas of study.
组织
包: The Epi阴道 kit VEC-100 contains 24 tissues, VEC-112 contains 12 tissues 和 VEC-606 contains 6 tissues. (组织“套件”包含组织, a small amount of culture Medium, 和 plasticware; contact 凯发体育最新版 for specific kit contents.)
基质: Single well tissue culture plate inserts are used.
vec - 100: Nunc™ single well tissue culture plate inserts. 孔径= 0.4µm，内径= 0.80厘米，表面积= 0.5 cm2.
vec - 606: Polycarbonate single well tissue culture plate inserts, Nunc™. 孔径= 0.4µm，内径= 2.5厘米，表面积= 4.2 cm2.
文化: 在气液界面.
组织学: vec - 100: 10-16 cell layers of non-cornified tissue.
很多数字: 每个技术员每周生产的组织批次被分配一个特定的批号. Typically, there are multiple lot numbers for any given week’s tissue production. 一个字母被附加在批号的末尾，以区分在给定的许多组织中的单个试剂盒. All tissue kits within a lot are identical with regard to cells, Medium, 处理, 培养条件, 等.
装运: At 4°C on Medium-supplemented, agarose gels.
装运: 周一.
交付: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location.
保质期: 包括运输时间在内，组织可在使用前在4°C保存4天. However, extended storage periods are not recommended unless absolutely necessary. 除了, 如果组织在同一天连续使用，可获得最好的重现性, e.g. Wednesday morning following overnight storage at 4°C.
长度的实验: 可持续培养至少1周，正常形态保持良好. Cultures must be fed every other day with 5.0 ml of maintenance Medium (vec - 100毫米). 细胞培养插入物放置在6孔板的培养架(部件号MEL-STND)或垫圈(部件号EPI-WSHR)上，以允许使用5个孔.0 ml.
替代组织:
VEC-100- ft:类似于VEC-100，除了在胶原/阴道成纤维细胞底物(固有层)上生长的组织。.
VEC-112-FT: Similar to VEC-100-FT except 12 tissues.
VLC-100: Similar to VEC-100 except tissues contain Langerhans cells.
VLC-112: Similar to VLC-100 except 12 tissues.
VLC-100- ft:类似于VLC-100，除了组织生长在胶原/阴道成纤维细胞底物(固有层)上。.
VLC-112-FT: Similar to VLC-100-FT except 12 tissues.
细胞
类型: 正常人子宫颈外细胞(NHEC)分化成无角化的组织, vaginal-ectocervical phenotype (part number VEC-100).
基因组成: 单一捐赠者.
来自: adult human ectocervical tissue.
筛选: HIV, Hepatitis-B, Hepatitis-C, mycoplasma, bacteria, yeast, fungi.
Medium
基础培养基: Dulbecco’s Modified Eagle’s Medium (DMEM) 和 F12 Medium.
生长因子/激素: Epidermal growth factor 和 other proprietary factors.
血清: 没有一个.
抗生素: Gentamicin 5 µg/ml (10% of normal gentamicin level).
抗真菌剂: 两性霉素B 0.25µg / ml.
酸碱指示剂: 酚红.
其他添加剂: 专有的.
选择: 酚红-free, antibiotic-free, anti-fungal-free Medium 和 tissue are available. Agents are removed 3 days prior to shipment.
维护中: vec - 100毫米
质量控制和无菌
目视检查: All tissues are visually inspected 和 if physical imperfections are noted, tissues are rejected for shipment.
最终用途测试: 组织 viability of each lot is determined by an MTT assay. 组织暴露于1.0% Triton X-100 for 30, 60, 和 120 minutes. 确定使用MTT法降低组织活力(ET-50)所需的暴露时间. The time of exposure needed to reduce the viability to 50% (ET-50) must be 0.92 – 1.67小时. 应客户要求，将QC测试结果汇总提供给客户参考. (For TEER measurements, 凯发体育最新版 has successfully used the WPI Endohm System.)
不育: All media used throughout the production process is checked for sterility. 维持培养基加抗生素和不加抗生素孵育1周，检查无菌情况. 来自24孔板的用于运输的琼脂糖凝胶也被培养1周，并检查任何污染的迹象.
筛查病原体: 对所有细胞进行筛选，并使用PCR检测艾滋病毒、乙型肝炎和丙型肝炎均为阴性. 然而，没有任何已知的测试方法可以完全保证这些细胞是无病原体的. 因此, 这些产品和所有人类衍生产品应按照CDC-NIH手册建议的BSL-2级别(生物安全级别2)或更高级别进行处理, “Biosafety in microbiological 和 biomedical laboratories,” 1998. 如需进一步协助，请联系您的现场安全主任或凯发体育最新版技术服务.
批不合格通知书: If a tissue lot fails our QC or sterility testing, 顾客会得到通知，并在适当的时候免费更换纸巾. Because some of our QC 和 sterility testing is done post-shipment, notification will be made as soon as possible. (Under normal circumstances, ET-50 failures will be notified by Thursday, 5 p.m.; sterility failures will be notified within 8 days of shipment.)
