Normal Human Dermal Fibroblasts

凯发体育最新版’s Normal Human Dermal Fibroblasts (NHDF) provide an optimized platform for investigating dermal biology.  Available from neonatal or adult donors across a variety of ethnicities, 凯发体育最新版’s NHDFs offer quality and convenience for the study of ECM production, wound healing, fibrosis, drug screening, and tissue modeling.

Donor matched keratinocytes (NHEK) & melanocytes (NHM) are available upon request.

凯发体育最新版’s NHDFs are isolated from neonatal (NHDF-CRY-NEO) or adult (NHDF-CRY-AD) donor tissue and cryopreserved.  When cultured according to 凯发体育最新版’s NHDF protocol, our fibroblasts are guaranteed to have ≥ 75% viability upon thaw and provide ≥ 15 population doublings.  NHDFs are characterized by their spindle morphology and expression of Vimentin.

Primary Human Dermal Fibroblasts
Primary Human Dermal Fibroblasts were stained for Vimentin (purple), Phalloidin (blue) and DAPI (yellow), 60x

Download the Human Dermal Fibroblasts Data Sheet

Download the NHDF Antioxidant Application Note

Specifications Table_NHDF

All cells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected by PCR for all donors and cell lots.  This product is for research use only.  Not for use in animals, humans or diagnostic purposes. 


凯发体育最新版’s cryopreserved human dermal fibroblasts are shipped on dry ice ($93 charge) every Monday, Tuesday, and Wednesday. Contact us at or +1-508-881-6771 to place an order.